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Determination of Zeaxanthin in New Food Raw Materials

Received: 10 May 2022     Published: 12 May 2022
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Abstract

The new food raw material zeaxanthin has been used in many food industries. However, the state has not yet formulated relevant detection methods for the detection of zeaxanthin raw material content. There are many reports on the extraction and purification of zeaxanthin in the existing literature, but there is no report on the method for the determination of zeaxanthin in the raw material of zeaxanthin. As a new food raw material, the content of zeaxanthin itself directly affects the content of the product. Therefore, developing a determination method of zeaxanthin in zeaxanthin raw material is urgently needed for the field of added zeaxanthin functional food research and product quality assurance. This study describes a method for the determination of zeaxanthin in new food raw materials by high performance liquid chromatography. Samples were dissolved with water and extracted with ultrasonication at a temperature range of 58–62°C, and then precipitated with mixed solvent of ethanol and trichloromethane. The supernatant was diluted by ethanoland and then filtered through a 0.45μm pore nylon filter, prior to injection into the HPLC system. Calibration of the chromatographic system was carried out by the external standard method. Analyses was carried out using an HPLC-UV Agilent 1260 system equipped with DP-4 gradient pump, and C30 column (250mm×4.6, 5μm) was used. The quantitative limit of zeaxanthin was 0.1g/100g and this method was accurate, repeatable, sensitivity.

Published in International Journal of Nutrition and Food Sciences (Volume 11, Issue 3)
DOI 10.11648/j.ijnfs.20221103.12
Page(s) 56-60
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2022. Published by Science Publishing Group

Keywords

Zeaxanthin, Liquid Chromatography, New Food Raw Materials, C30 Column

References
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Cite This Article
  • APA Style

    Duan Guoxia, Liu Lijun, Chen Shumin, Chen Jing, Liu Chunxia, et al. (2022). Determination of Zeaxanthin in New Food Raw Materials. International Journal of Nutrition and Food Sciences, 11(3), 56-60. https://doi.org/10.11648/j.ijnfs.20221103.12

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    ACS Style

    Duan Guoxia; Liu Lijun; Chen Shumin; Chen Jing; Liu Chunxia, et al. Determination of Zeaxanthin in New Food Raw Materials. Int. J. Nutr. Food Sci. 2022, 11(3), 56-60. doi: 10.11648/j.ijnfs.20221103.12

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    AMA Style

    Duan Guoxia, Liu Lijun, Chen Shumin, Chen Jing, Liu Chunxia, et al. Determination of Zeaxanthin in New Food Raw Materials. Int J Nutr Food Sci. 2022;11(3):56-60. doi: 10.11648/j.ijnfs.20221103.12

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  • @article{10.11648/j.ijnfs.20221103.12,
      author = {Duan Guoxia and Liu Lijun and Chen Shumin and Chen Jing and Liu Chunxia and Wu Lunwei and Gong Huili and Yue Hong and Liu Wang and Li Cuizhi and Lv Zhiyong},
      title = {Determination of Zeaxanthin in New Food Raw Materials},
      journal = {International Journal of Nutrition and Food Sciences},
      volume = {11},
      number = {3},
      pages = {56-60},
      doi = {10.11648/j.ijnfs.20221103.12},
      url = {https://doi.org/10.11648/j.ijnfs.20221103.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20221103.12},
      abstract = {The new food raw material zeaxanthin has been used in many food industries. However, the state has not yet formulated relevant detection methods for the detection of zeaxanthin raw material content. There are many reports on the extraction and purification of zeaxanthin in the existing literature, but there is no report on the method for the determination of zeaxanthin in the raw material of zeaxanthin. As a new food raw material, the content of zeaxanthin itself directly affects the content of the product. Therefore, developing a determination method of zeaxanthin in zeaxanthin raw material is urgently needed for the field of added zeaxanthin functional food research and product quality assurance. This study describes a method for the determination of zeaxanthin in new food raw materials by high performance liquid chromatography. Samples were dissolved with water and extracted with ultrasonication at a temperature range of 58–62°C, and then precipitated with mixed solvent of ethanol and trichloromethane. The supernatant was diluted by ethanoland and then filtered through a 0.45μm pore nylon filter, prior to injection into the HPLC system. Calibration of the chromatographic system was carried out by the external standard method. Analyses was carried out using an HPLC-UV Agilent 1260 system equipped with DP-4 gradient pump, and C30 column (250mm×4.6, 5μm) was used. The quantitative limit of zeaxanthin was 0.1g/100g and this method was accurate, repeatable, sensitivity.},
     year = {2022}
    }
    

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  • TY  - JOUR
    T1  - Determination of Zeaxanthin in New Food Raw Materials
    AU  - Duan Guoxia
    AU  - Liu Lijun
    AU  - Chen Shumin
    AU  - Chen Jing
    AU  - Liu Chunxia
    AU  - Wu Lunwei
    AU  - Gong Huili
    AU  - Yue Hong
    AU  - Liu Wang
    AU  - Li Cuizhi
    AU  - Lv Zhiyong
    Y1  - 2022/05/12
    PY  - 2022
    N1  - https://doi.org/10.11648/j.ijnfs.20221103.12
    DO  - 10.11648/j.ijnfs.20221103.12
    T2  - International Journal of Nutrition and Food Sciences
    JF  - International Journal of Nutrition and Food Sciences
    JO  - International Journal of Nutrition and Food Sciences
    SP  - 56
    EP  - 60
    PB  - Science Publishing Group
    SN  - 2327-2716
    UR  - https://doi.org/10.11648/j.ijnfs.20221103.12
    AB  - The new food raw material zeaxanthin has been used in many food industries. However, the state has not yet formulated relevant detection methods for the detection of zeaxanthin raw material content. There are many reports on the extraction and purification of zeaxanthin in the existing literature, but there is no report on the method for the determination of zeaxanthin in the raw material of zeaxanthin. As a new food raw material, the content of zeaxanthin itself directly affects the content of the product. Therefore, developing a determination method of zeaxanthin in zeaxanthin raw material is urgently needed for the field of added zeaxanthin functional food research and product quality assurance. This study describes a method for the determination of zeaxanthin in new food raw materials by high performance liquid chromatography. Samples were dissolved with water and extracted with ultrasonication at a temperature range of 58–62°C, and then precipitated with mixed solvent of ethanol and trichloromethane. The supernatant was diluted by ethanoland and then filtered through a 0.45μm pore nylon filter, prior to injection into the HPLC system. Calibration of the chromatographic system was carried out by the external standard method. Analyses was carried out using an HPLC-UV Agilent 1260 system equipped with DP-4 gradient pump, and C30 column (250mm×4.6, 5μm) was used. The quantitative limit of zeaxanthin was 0.1g/100g and this method was accurate, repeatable, sensitivity.
    VL  - 11
    IS  - 3
    ER  - 

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Author Information
  • Inner Mongolia Yili Industrial Group Company Limited, Hohhot, China

  • Inner Mongolia Yili Industrial Group Company Limited, Hohhot, China

  • Inner Mongolia Yili Industrial Group Company Limited, Hohhot, China

  • Inner Mongolia Yili Industrial Group Company Limited, Hohhot, China

  • Inner Mongolia Yili Industrial Group Company Limited, Hohhot, China

  • Inner Mongolia Yili Industrial Group Company Limited, Hohhot, China

  • Inner Mongolia Yili Industrial Group Company Limited, Hohhot, China

  • Inner Mongolia Yili Industrial Group Company Limited, Hohhot, China

  • Inner Mongolia Yili Industrial Group Company Limited, Hohhot, China

  • Inner Mongolia Yili Industrial Group Company Limited, Hohhot, China

  • Inner Mongolia Yili Industrial Group Company Limited, Hohhot, China

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