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Protective Effects of Pre-Germinated Brown Rice Extract against Amyloid β-Peptide Induced Neurotoxicity in Neuronal SK-N-SH Cells

Published: 20 June 2013
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Abstract

Amyloid β-peptide (Aβ1-42) is the major component of amyloid plaques and is believed to induce oxidative stress and inflammation in brain, which are postulated to play important roles in the pathogenesis of Alzheimer’s disease (AD). The present study investigated the effect of pre-germinated brown rice extract (Oryza sativa L., PGBR ex) which contains a much higher concentration of essential amino acids, such as lysine, isoleucine, methionine and several kinds of antioxidants than conventional brown rice, and over 8-13 times the amount of γ-aminobutyric acid (GABA) shown to protect against oxidative stress and cell death. Pre-treatment of SK-N-SH cells with 2000 μg/ml PGBR ex significantly attenuated 10 μM Aβ1-42-induced neuronal cells deaths. Apoptotic cells death was demonstrated morphologically as well as by detection of the presence of activated caspase-3, DNA fragmentation and the production of reactive oxygen species (ROS) which could be reduced by pre-treatment of PGBR ex and brown rice extract (BR ex). These results suggest that the protective effect of PGBR ex and BR ex against Aβ1-42-induced neuronal cell death might be due to its anti-oxidative and anti-apoptotic properties and could be useful as a value added functional food to prevent neurodegenerative diseases.

Published in International Journal of Nutrition and Food Sciences (Volume 2, Issue 4)
DOI 10.11648/j.ijnfs.20130204.12
Page(s) 167-173
Creative Commons

This is an Open Access article, distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution and reproduction in any medium or format, provided the original work is properly cited.

Copyright

Copyright © The Author(s), 2013. Published by Science Publishing Group

Keywords

Brown Rice Extract, SK-N-SH Cells, Antioxidant, GABA, ROS, Aβ1-42, Apoptosis, DNA Fragmentation

References
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Cite This Article
  • APA Style

    Rungtip Soi-ampornkul, Chatchawan Srisawat, Wipawan Thangnipon, Surin Kanyok, Sarawut Junnu, et al. (2013). Protective Effects of Pre-Germinated Brown Rice Extract against Amyloid β-Peptide Induced Neurotoxicity in Neuronal SK-N-SH Cells. International Journal of Nutrition and Food Sciences, 2(4), 167-173. https://doi.org/10.11648/j.ijnfs.20130204.12

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    ACS Style

    Rungtip Soi-ampornkul; Chatchawan Srisawat; Wipawan Thangnipon; Surin Kanyok; Sarawut Junnu, et al. Protective Effects of Pre-Germinated Brown Rice Extract against Amyloid β-Peptide Induced Neurotoxicity in Neuronal SK-N-SH Cells. Int. J. Nutr. Food Sci. 2013, 2(4), 167-173. doi: 10.11648/j.ijnfs.20130204.12

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    AMA Style

    Rungtip Soi-ampornkul, Chatchawan Srisawat, Wipawan Thangnipon, Surin Kanyok, Sarawut Junnu, et al. Protective Effects of Pre-Germinated Brown Rice Extract against Amyloid β-Peptide Induced Neurotoxicity in Neuronal SK-N-SH Cells. Int J Nutr Food Sci. 2013;2(4):167-173. doi: 10.11648/j.ijnfs.20130204.12

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  • @article{10.11648/j.ijnfs.20130204.12,
      author = {Rungtip Soi-ampornkul and Chatchawan Srisawat and Wipawan Thangnipon and Surin Kanyok and Sarawut Junnu and Wanphen Katanyoo and Sompong Liammongkolkul and Pairat Soi-ampornkul},
      title = {Protective Effects of Pre-Germinated Brown Rice Extract against Amyloid β-Peptide Induced Neurotoxicity in Neuronal SK-N-SH Cells},
      journal = {International Journal of Nutrition and Food Sciences},
      volume = {2},
      number = {4},
      pages = {167-173},
      doi = {10.11648/j.ijnfs.20130204.12},
      url = {https://doi.org/10.11648/j.ijnfs.20130204.12},
      eprint = {https://article.sciencepublishinggroup.com/pdf/10.11648.j.ijnfs.20130204.12},
      abstract = {Amyloid β-peptide (Aβ1-42) is the major component of amyloid plaques and is believed to induce oxidative stress and inflammation in brain, which are postulated to play important roles in the pathogenesis of Alzheimer’s disease (AD). The present study investigated the effect of pre-germinated brown rice extract (Oryza sativa L., PGBR ex) which contains a much higher concentration of essential amino acids, such as lysine, isoleucine, methionine and several kinds of antioxidants than conventional brown rice, and over 8-13 times the amount of γ-aminobutyric acid (GABA) shown to protect against oxidative stress and cell death. Pre-treatment of SK-N-SH cells with 2000 μg/ml PGBR ex significantly attenuated 10 μM Aβ1-42-induced neuronal cells deaths. Apoptotic cells death was demonstrated morphologically as well as by detection of the presence of activated caspase-3, DNA fragmentation and the production of reactive oxygen species (ROS) which could be reduced by pre-treatment of PGBR ex and brown rice extract (BR ex). These results suggest that the protective effect of PGBR ex and BR ex against Aβ1-42-induced neuronal cell death might be due to its anti-oxidative and anti-apoptotic properties and could be useful as a value added functional food to prevent neurodegenerative diseases.},
     year = {2013}
    }
    

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  • TY  - JOUR
    T1  - Protective Effects of Pre-Germinated Brown Rice Extract against Amyloid β-Peptide Induced Neurotoxicity in Neuronal SK-N-SH Cells
    AU  - Rungtip Soi-ampornkul
    AU  - Chatchawan Srisawat
    AU  - Wipawan Thangnipon
    AU  - Surin Kanyok
    AU  - Sarawut Junnu
    AU  - Wanphen Katanyoo
    AU  - Sompong Liammongkolkul
    AU  - Pairat Soi-ampornkul
    Y1  - 2013/06/20
    PY  - 2013
    N1  - https://doi.org/10.11648/j.ijnfs.20130204.12
    DO  - 10.11648/j.ijnfs.20130204.12
    T2  - International Journal of Nutrition and Food Sciences
    JF  - International Journal of Nutrition and Food Sciences
    JO  - International Journal of Nutrition and Food Sciences
    SP  - 167
    EP  - 173
    PB  - Science Publishing Group
    SN  - 2327-2716
    UR  - https://doi.org/10.11648/j.ijnfs.20130204.12
    AB  - Amyloid β-peptide (Aβ1-42) is the major component of amyloid plaques and is believed to induce oxidative stress and inflammation in brain, which are postulated to play important roles in the pathogenesis of Alzheimer’s disease (AD). The present study investigated the effect of pre-germinated brown rice extract (Oryza sativa L., PGBR ex) which contains a much higher concentration of essential amino acids, such as lysine, isoleucine, methionine and several kinds of antioxidants than conventional brown rice, and over 8-13 times the amount of γ-aminobutyric acid (GABA) shown to protect against oxidative stress and cell death. Pre-treatment of SK-N-SH cells with 2000 μg/ml PGBR ex significantly attenuated 10 μM Aβ1-42-induced neuronal cells deaths. Apoptotic cells death was demonstrated morphologically as well as by detection of the presence of activated caspase-3, DNA fragmentation and the production of reactive oxygen species (ROS) which could be reduced by pre-treatment of PGBR ex and brown rice extract (BR ex). These results suggest that the protective effect of PGBR ex and BR ex against Aβ1-42-induced neuronal cell death might be due to its anti-oxidative and anti-apoptotic properties and could be useful as a value added functional food to prevent neurodegenerative diseases.
    VL  - 2
    IS  - 4
    ER  - 

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Author Information
  • Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok,Thailand

  • Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok,Thailand

  • Research Center for Neuroscience, Institute of Molecular Biosciences, Mahidol University, Salaya, Nakhonpathom, Thailand

  • Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok,Thailand

  • Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok,Thailand

  • Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok,Thailand

  • Department of Biochemistry, Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok,Thailand

  • Surasak montree school, The Ministry of Education, Wipawadee road, Huaikhwang, Bangkok, Thailand

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